Monkeypox Virus Detection—qPCR & Rapid PCR

Part 01 Monkeypox Outbreaks
Monkeypox, a disease rarely seen outside of Central and West African countries, infections were confirmed in Italy, Sweden, Spain, Portugal, United States, Canada, and United Kingdom, which has been hot spots recently. According to WHO, as of May 22nd, 2022, there were approximately 92 confirmed cases of monkeypox worldwide, with many more cases still to be confirmed. More cases are likely to be identified in the future as surveillance is expanded.
Part 02 Highly contagious, zoonotic
Monkeypox virus was first discovered in 1958 when a “pox-like” infection developed in a group of monkeys used for research, hence the name. It is a zoonotic virus that can infect a wide range of animals in nature, along with the once feared smallpox virus. The virus is transmitted to humans mainly through the skin, respiratory tract or wounds around the eyes or mucous membranes of the nose and mouth of infected animals. Human-to-human transmission is mainly caused by close contact with respiratory secretions, diseased skin, or objects contaminated with body fluids or diseased tissue from an infected person. Transmission by respiratory droplets usually requires prolonged face-to-face contact.
Part 03 High lethality, Early diagnosis is required
Initial symptoms of monkeypox virus infection in humans include fever, headache, muscle aches, back pain and swollen lymph nodes, which can then progress to a widespread rash on the face and body. Typically, monkeypox is a self-limiting disease with symptoms lasting between 2 and 4 weeks. Most infected people recover within a few weeks, but there are cases of severe illness and death. The death rate from monkeypox has historically ranged from 0% to 11% in the general population, and has been higher in young children. In recent years, the mortality rate has been around 3-6%. Furthermore, some of the symptoms of monkeypox are similar to those of other diseases that present with a rash, which can easily lead to misdiagnosis. As of today, there is still no effective treatment for monkeypox in humans. Therefore, early diagnosis is one of the most effective means of prevention and control, and accurate detection and early diagnosis of monkeypox virus is essential for disease identification and control.
Part 04 Quick Strike By TargetingOne
Relying on the international leading molecular diagnostic technology platform and experience of SARS CoV 2 and various infectious pathogens, TargetingOne research team has rapidly developed Monkeypox virus nucleic acid real-time PCR detection kit, which can rapidly identify Monkeypox virus by specifically detecting the nucleic acid fragments of Monkeypox virus. The reagent is highly sensitive and simple to use. Our real-time PCR kits are uniquely designed with a lyophilized format, this affords our customers more convenient shipping and storage options.

Monkeypox virus nucleic acid real-time PCR detection kit
Detection principle: Using specific primers of monkeypox virus in the reagents and monkeypox virus genes in samples amplified by fluorescent probe PCR, and generate the fluorescent signals by the PCR amplification for accurate qualitative detection of monkeypox virus nucleic acids.
Sample type: Nasal swabs, pharyngeal swabs, blood and other clinical samples
Suitable instruments: TargetingOne C1, ABI7500, 7500FAST, Roche z480, Biorad CFX96, etc.
Product features:
● Easy to operate, only two steps are needed: “add sample” and “test on machine”; also suitable for no extraction and routine extraction
●Short reaction time, 30 minutes for our TargetingOne C1 and less than 60 minutes for other instruments
● Lyophilised reagents for easy transport and storage
● Human-derived internal standard for monitoring the collection, monitor the transport and extraction of the sample to ensure the accuracy of the results

Rapid PCR Solution Project Introduction

The Monkeypox virus nucleic acid detection kit adopts real-time fluorescent PCR technology to detect the conserved regions of the Monkeypox virus with high specificity based on TaqMan fluorescent probes. The kit is also equipped with primers and probes for the detection of the internal reference gene, which allows for quality control of the sampling, extraction, and reverse transcription PCR steps to ensure the accuracy and reliability of the detection process. Equipped with easyPCR C1, a portable fast real-time fluorescence PCR analyzer from TargetingOne, enables rapid and accurate monkeypox virus-related assays in multiple scenarios with superior temperature control, optical technology, easy-to-operate software, and a lightweight portable body, breaking through the limitations of laboratory space.