Cat.No.: 30075
The fluorescent PCR reaction system is microtitrated by microfluidic technology to randomly distribute target nucleic acid molecules into separate and highly homogeneous water-in-oil (W/O) droplets. The target nucleic acid sequences are randomly distributed into separate and highly homogeneous water-in-oil (W/O) microdroplets, which are amplified to the endpoint by PCR. The PCR amplification to the endpoint enables absolute quantification of the target nucleic acid sequence by molecular counting and is not dependent on the standard curve. As the latest generation of nucleic acid quantification methods, its technical features include higher detection sensitivity, data precision and resolution, higher repeatability and reproducibility, and low sensitivity to changes in PCR inhibitors and PCR amplification efficiency.
